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Xiaolong Luo Mail: 5108 Huron Street, College Park, MD 20740 C.V. is also available in PDF and DOC.
Email: xlluo@umd.edu
URL: www.glue.umd.edu/~xlluo
Tel: 301-405-2971 (O); 301-405-0694 (Lab)
Accomplishments
  1. Developed bioMEMS for programmable in situ biomolecule/nanoentity assembly with (1) electrical / optical access, (2) non-permanent sealing and reusability, and (3) dead-volume free packaging and cross-channel design to minimize non-specific binding
  2. Utilized polysaccharide chitosan to (1) biofunctionalize bioMEMS with spatial, temporal and biochemical programmability, and (2) assemble proteins and hybridize DNA in bioMEMS
  3. Developed a microfluidic control system from design, LabView programming to hardware integration to control the selection and pumping of different fluid sources into microchannels
  4. Demonstrated, for the first time, programmable assembly of a metabolic pathway enzyme, Pfs, and its catalytic activity at specific sites in bioMEMS, which (1) established a versatile technology to study bacteria communication, and (2) emerged a novel screening strategy for antimicrobial drugs
  5. Assembled GHLPT (protein G-His6-LuxS-Pfs-Tyr5) nanofactories and E. Coli cells in bioMEMS for in vitro study of Quorum Sensing (QS) metabolic pathway and biofilm formation
  6. Microfabricated active sites for Surface Enhanced Raman Spectrometry (SERS) in bioMEMS for in situ detection and quantification of biochemical reaction products
  7. Synthesized vertical, permeable chitosan membrane in microchannels as microreactor for enhanced enzymatic conversion efficiency
Education
Ph.D. in Bioengineering, University of Maryland, College Park, MD Advisor: Dr. Gary W. Rubloff
2008

Thesis: Programmable biomolecule assembly and activity in prepackaged bioMEMS
M.S. in Mechanical Engineering, Temple University, Philadelphia, PA Advisor: Dr. Jim S. Chen
2003
  Thesis: PIV measurement and CFD simulation of flow field in a human uterus model for cryoablation
B.S. in Mechatronics (five-year program), Zhejiang University, Hangzhou, China Advisor: Dr. Minxiu Qiu
2001
  Thesis: Design and dynamic simulation of an electro-hydraulic pressure control valve
       
Awards and honors
Deutsch Fellow, Deutsch Foundation, University of Maryland, College Park, MD
2006 ~ 2008

3rd Place Student Research Award, BIOMATERIALS INTERFACE DIVISION, 53rd Annual AVS International Symposium, San Francisco, CA, Nov. 12-17, 2006
Nov. 2006
Graduate School Fellowship, University of Maryland, College Park, MD
2003 ~ 2005
Bioengineering Program Fellowship, University of Maryland, College Park, MD
2003 ~ 2004
3 Academic Excellent Scholarships, Zhejiang University, Hangzhou, China
1997 ~ 2000
3 Honor Awards, Zhejiang University, Hangzhou, China
1997 ~ 2000
Experience
Research Assistantships  
  Nano-Bio-Systems Lab (NBSL), University of Maryland, College Park, MD 08/2004~present
  Achieved: Programmable biomolecule assembly and activity in bioMEMS  
   
  • Developed bioMEMS and control system for biomolecule assembly and with spatial, temporal and biochemical programmability
  
 
  • Assembled model protein (GFP), DNA, single metabolic pathway enzyme (Pfs), integrated enzyme complex and E. coli cells at specific sites in bioMEMS
 
  Ongoing: Metabolic pathway reconstruction and interrogation in bioMEMS  
   
  • Reconstruct the autoinducer-2 biosynthesis pathway of multiple enzymes
  
   
  • Screen the inhibitors of AI-2 synthases as antimicrobial drug candidates

 
   
  • Detect and quantify biochemical reaction products in real-time with SERS
  
       
Human Performance Lab, University of Maryland, College Park, MD 07/2003~08/2003
  • Modeling and simulation of internal body temperature at work and rest
  
 
Thermal Processing Laboratory, Temple University, Philadelphia, PA 08/2002~06/2003
  • Applying Particle Image Velocimetry (PIV) and Computational Fluidic Dynamics (CFD) to
    model the fluidic flow fields inside a human uterus during cryo-ablation
  
The State Key Lab of Fluid Power Transmission and Control, Zhejiang University, China 01/2001~06/2001
  • Designing, optimizing and simulating an electrohydraulic pressure control valve
  
The Institute of Mechatronic Control Engineering, Zhejiang University, China 06/1999~11/1999
 

Teaching Assistantships

  
Fischell Department of Bioengineering, University of Maryland, College Park, MD  
  1. Introduction to Biological Materials
  2. Cell Electrophysiology
 Instructor: Dr. Seog, Joonil
Instructor: Dr. Keith E. Herold		 08/2007~12/2007
01/2005~05/2005
     
  Department of Mechanical Engineering, Temple University, Philadelphia, PA  
   
  1. Engineering Graphics (AutoCAD)
  2. Numerically Controlled Machinery Lab (CNC)
  3. Mechanics of Fluids
 Instructor: Dr. Vallorie Peridier
Instructor: Mr. Mustafa Ayranci
Instructor: Dr. Jim S. Chen		 08/2001~12/2002
01/2002~05/2002
08/2002~12/2002
Skills
Microfabrication bioMEMS/Microfluidics, solid lithography (SU-8, metal), soft lithography (PDMS), metal sputtering/evaporation/electroplating, RIE, profilometry, bioMEMS packaging, oxygen plasma bonding
Other lab skill liquid chromatography (HPLC), affinity chromatography, Particle Image Velocimetry (PIV), Raman spectrometry, plasmid construction, protein purification, enzyme conjugation
Computer software AutoCAD, COMSOL, MatLab, ANSYS, LabVIEW, Origin, Visio, VisSim, Dreamweaver
Language Fluent in English and Chinese
Activities
 
Student member of Materials Research Society (MRS)
  Biomedical Engineering Society (BMES)
  American Vacuum Society (AVS)
  American Association for the Advancement of Science ( AAAS)
President of  Maryland Chinese Bible Study Group, 2008~2009
Publications
Journal Articles (EndNote Library)
  1. J. J. Park, X. L. Luo, H. M. Yi, T. M. Valentine, G. F. Payne, W. E. Bentley, R. Ghodssi and G. W. Rubloff, “Chitosan-mediated in situ biomolecule assembly in completely packaged microfluidic devices,” Lab on a Chip, 2006, 6, pp. 1315-1321. [pdf]
  2. A. T. Lewandowski, H. M. Yi, X. L. Luo, G. F. Payne, R. Ghodssi, G. W. Rubloff and W. E. Bentley, “Protein assembly onto patterned microfabricated devices through enzymatic activation of fusion pro-tag,” Biotechnology and Bioengineering, 2008, 99, pp. 499-507. [pdf]
  3. X. L. Luo, A. T. Lewandowski, H. M. Yi, R. Ghodssi, G. F. Payne, W. E. Bentley and G. W. Rubloff, “Programmable assembly of a metabolic pathway enzyme in a pre-packaged reusable bioMEMS device,” Lab on a Chip, 2008, 8, pp. 420-430, [pdf].
  4. X. L. Luo, D. Larios Berlin, S. B. Buckhout-White, R. Ghodssi and G. W. Rubloff, “Design optimization for bioMEMS studies of enzyme-controlled metabolic pathways,” Biomedical Microdevices, in press, DOI 10.1007/s10544-008-9204-5.
  5. R. Fernandes*, X. L. Luo*, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Spatially localized quorum sensing in a microfluidic device,” in preparation. (*co-first authors)
  6. X. L. Luo, S. B. Buckhout-White, D. Larios Berlin and G. W. Rubloff, “Chitosan-mediated facile fabrication of nano-roughened structures for SERS measurement,” in preparation.
  7. X. L. Luo, D. Larios Berlin, J. Gair and G. W. Rubloff, “In situ synthesis and applications of vertical chitosan membrane in bioMEMS,” in preparation.
  8. X. L. Luo, H. M. Yi, J. Culver and G. W. Rubloff, “Selective assembly of nanoentities in bioMEMS,” in preparation.
Papers in Refereed Conference Proceedings
  1. X. L. Luo, J. S. J. Chen, M. Wolfson, C. Philips and T. Shaffer, “PIV measurement of fluid flow inside a human uterus model for cryoablation,” Proceedings of IEEE 29th Annual Bioengineering Conference, Newark, NJ, March 22-23, 2003, pp. 225-226. [pdf]
  2. J. J. Park, X. L. Luo, H. Yi, R. Ghodssi and G.W. Rubloff, “In situ Biomolecule Assembly and Activity within Completely Packaged Microfluidic Devices,” Proceedings of IEEE/NLM Life Science Systems and Applications Workshop, Bethesda, MD, July 13-14, 2006, pp. 88-89. [pdf]
  3. X. L. Luo, J. J. Park, H. Yi, A. T. Lewandowski, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, “Chitosan-mediated Enzyme Assembly toward Rebuilding a Metabolic Pathway in the Microfluidic Environment,” Proceedings of Materials Research Society 2007 Spring Meeting, San Francisco, CA, April 9-13, 2007. [pdf]
  4. X. L. Luo, A. T. Lewandowski, H. Yi, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Signal-Guided Sequential Assembly of Nano-Bio-Components in a Completely Packaged Microfluidic Environment,” Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Paris, France, October 7-11, 2007, pp. 784-786.
  5. X. L. Luo, A. T. Lewandowski, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Enzyme Assembly and Catalytic Activity in a Reusable BioMEMS Plateform for Metabolic Engineering,” Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Paris, France, October 7-11, 2007, pp. 1616-1618.
  6. X. L. Luo, A. T. Lewandowski, D. Berlin, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Applications and Improvements of BioMEMS for Chitosan-mediated Enzyme Assembly and Catalytic Activity,” Proceedings of the First Shenyang International Colloquium on Microfluidics (SICOM I), Shenyang, China. October 21-24, 2007, pp. 204-207.
  7. S. Buckout-White, X. L. Luo, D. Larios Berlin, G. F. Payne, W. E. Bentley, R. Ghodssi and G. W. Rubloff, "A Platform for In-situ Sensing of Biomolecules Using a Chitosan Mediated SERS Substrate Fabricated in Microfluidics," Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), San Diago, CA, USA, October 12-16, 2008. Accepted.
  8. X. L. Luo, D. Larios Berlin, S. Buckout-White, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "Minimizing Parasitic Reactions for Enyme-Controlled Metabolic Pathways Investigated in BioMEMS," Proceedings of the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), San Diago, CA, USA, October 12-16, 2008. Accepted.
Thesis
  1. X. L. Luo, "Design and dynamic simulation of an electro-hydraulic pressure control valve," Bachelor of Engineering thesis, Zhejiang University, Hangzhou, Zhejiang, China, 2001. [pdf] (In Chinese)
  2. X. L. Luo, "PIV measurement and CFD simulation of flow field in a human uterus model for cryoablation," Master of Science and Engineering thesis, Temple University, Philadelphia, PA, 2003. [pdf]
  3. X. L. Luo, "Programmable biomolecule assembly and activity in prepackaged bioMEMS," Doctor of Philosophy, University of Maryland, College Park, MD, 2008. [pdf]
Conference talks and posters (Presenting author underlined)
  1. X. L. Luo, J. S. J. Chen, M. Wolfson, C. Philips and T. Shaffer, “PIV measurement of fluid flow inside a human uterus model for cryoablation,” IEEE 29th Annual Bioengineering Conference, Newark, NJ, March 22-23, 2003. (Poster presentation)
  2. J. J. Park, X. L. Luo, H. Yi, R. Ghodssi and G.W. Rubloff, “In situ Biomolecule Assembly and Activity within Completely Packaged Microfluidic Devices,” IEEE/NLM Life Science Systems and Applications Workshop, Bethesda, MD, July 13-14, 2006. (Oral presentation)
  3. X. L. Luo, J. J. Park, H. Yi, R. Ghodssi and G. W. Rubloff, “Biomolecule Assembly and Functionality in Completely Packaged Microfluidic Devices,” American Vacuum Society 53rd International Symposium, San Francisco, CA, November 12-17, 2006. (Oral presentation)
  4. X. L. Luo, J. J. Park, H. Yi, A. T. Lewandowski, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, “Chitosan-mediated Enzyme Assembly toward Rebuilding a Metabolic Pathway in the Microfluidic Environment,” Materials Research Society 2007 Spring Meeting, San Francisco, CA, April 9-13, 2007. (Oral presentation)
  5. X. L. Luo, A. T. Lewandowski, H. Yi, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Signal-Guided Sequential Assembly of Nano-Bio-Components in a Completely Packaged Microfluidic Environment,” the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Paris, France, October 7-11, 2007. (Poster presentation)
  6. X. L. Luo, A. T. Lewandowski, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Enzyme Assembly and Catalytic Activity in a Reusable BioMEMS Platform for Metabolic Engineering,” the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), Paris, France, October 7-11, 2007. (Poster presentation)
  7. X. L. Luo, A. T. Lewandowski, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Reversible Biofunctionalization and Catalytic Activity of a Metabolic Pathway Enzyme in Reusable BioMEMS Devices,” American Vacuum Society 54th International Symposium, Seattle, WA, October 14-19, 2007. (Oral presentation)
  8. X. L. Luo, A. T. Lewandowski, D. Berlin, G. F. Payne, R. Ghodssi, W. E. Bentley and G. W. Rubloff, “Applications and Improvements of BioMEMS for Chitosan-mediated Enzyme Assembly and Catalytic Activity,” First Shenyang International Colloquium on Microfluidics (SICOM I), Shenyang, China. October 21-24, 2007. (Oral presentation)
  9. X. L. Luo, D. Larios Berlin, J. Gair and G. W. Rubloff, "Microfabrication and Biofunctionalization of Permeable Vertical Chitosan Membranes in Microfluidics," BMES 2008 Annual Fall Meeting, St. Louis, MO, October 2-4, 2007. To appear. (Poster presentation)
  10. X. L. Luo, D. Larios Berlin, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "BioMEMS Study and Design Optimization of In Vitro Enzyme-Controlled Metabolic Pathways," BMES 2008 Annual Fall Meeting, St. Louis, MO, October 2-4, 2007. To appear. (Oral presentation)
  11. X. L. Luo, D. Larios Berlin, S. Buckout-White, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "Minimizing Parasitic Reactions for Enyme-Controlled Metabolic Pathways Investigated in BioMEMS," the 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS), San Diago, CA, USA, October 12-16, 2008. To appear. (Poster presentation)
  12. X. L. Luo, D. Larios Berlin, W. E. Bentley, G. F. Payne, R. Ghodssi and G. W. Rubloff, "Avoiding Parasitic Reactions Due to Interconnect Dead Volume and Non-Specific Binding in Microfluidics," American Vacuum Society 54th International Symposium, Boston, MA, October 19-24, 2007. (Oral presentation)

This page was last updated on 07/03/08.